WebOptogenetics is a biological technique to control the activity of neurons or other cell types with light. This is achieved by expression of light-sensitive ion channels, pumps or enzymes specifically in the target cells. On the level of individual cells, light-activated enzymes and transcription factors allow precise control of biochemical signaling pathways. WebDownloadable! Active nucleocytoplasmic transport is a key mechanism underlying protein regulation in eukaryotes. While nuclear protein import can be controlled in space and time with a portfolio of optogenetic tools, protein export has not been tackled so far. Here we present a light-inducible nuclear export system (LEXY) based on a single, genetically …
Analysis of Slow-Cycling Variants of the Light-Inducible Nuclear ...
WebMar 1, 2013 · Nuclear Export of Htt Depends on Exportin 1 . Nuclear export of proteins by a leucine-rich NES is mediated by Crm1 in yeast and exportin 1 in mammalian cells (26, 27). A ternary complex consisting of the NES cargo, Ran-GTP, and Crm1/exportin 1 is formed in the nucleus and subsequently translocates out of the nucleus through the nuclear pore. WebOptogenetic control of nuclear protein import and export and gene expression in living cells. ( A and B) Representative fluorescence images of U2OS cells transiently transfected with NES-mCherry-LINuS (A) and NLS-mCherry-LEXY (B) before (left image) and after (right image) 8 min of blue light illumination. opal 2 sheffield
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WebOct 5, 2024 · To measure H2B ubiquitylation and deubiquitination kinetics in vivo, we used a rapid and reversible optogenetic tool, the light-inducible … WebFeb 8, 2016 · While nuclear protein import can be controlled in space and time with a portfolio of optogenetic tools, protein export has not been tackled so far. Here we present … WebJun 2, 2016 · The light-inducible nuclear localization signal (LINuS) was developed based on a natural plant photoreceptor that reversibly triggers the import of proteins of interest into … iowa dnr tournaments