Germacrene synthase

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August undefined, 2024

WebNov 22, 2013 · An additional sesquiterpene synthase (germacrene A synthase) has been cloned from A. annua but this enzyme was not included in the promoter studies. The cloned promoters have been fused to the GUS reporter gene and transgenic A. annua plants expressing these fusions have been established. Here we report on studies on the … WebGermacrene D Receptor Neurons. One type of receptor neurons, tuned to the sesquiterpene germacrene D, frequently occurred in recordings from heliothine moths, and was the first type identified in these species57 ( Fig. 10.1 ). The response was elicited by mixtures obtained from many plant species, both hosts and non-hosts.

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WebMay 18, 2024 · Germacrene D was derived from the linear C15 precursor farnesyl diphosphate (FPP) through highly complex reactions catalyzed by germacrene D … WebDec 15, 2004 · Two new sesquiterpene synthase cDNAs, designated Sc11 and Sc19, have been isolated from goldenrod and functional expression in Escherichia coli identified Sc11 as (+)-germacrene D synthase and Sc19 as (-)-germacrene D synthase. Thus, the enantiomers of germacrene D are the products of separate, but closely related (85% … running shoes for men saucony

germacrene D synthase - Wikipedia

WebDec 17, 2015 · Germacrene A synthase (GAS) from Solidago canadensis catalyzes the conversion of farnesyl diphosphate (FDP) to the plant sesquiterpene (+)-germacrene A. After diphosphate expulsion, farnesyl cation reacts with the distal 10,11-double bond to afford germacrene A (>96%) and 2% α-humulene, which arises from 1,11-cyclization of … WebJul 16, 2024 · The genes underlying STL biosynthesis have been partially characterized. Genes encoding the enzyme catalysing the first step of the STL production, germacrene … WebOct 24, 2024 · a germacrene synthase from dandelion. Subsequently, biosynthesis of signiﬁcant amounts of. germacrene-A was observed in the most productive strain compared to the wild type. Thus, sccm reporting email

Isolation and Characterization of Two Germacrene A Synthase …

Frontiers Multi-Substrate Terpene Synthases: Their Occurrence …

WebMay 22, 2024 · Germacrene A synthase (GAS) catalyses a 1,10-cyclisation of FPP to B, followed by deprotonation to 1 (Scheme 3). Both enantiomers of 1 are accessible through this reaction, depending on whether C10 of … sccm reporting permissionsWebJan 7, 2024 · Background: The sesquiterpene germacrene A is a direct precursor of ß-elemene that is a major component of the Chinese medicinal herb Curcuma wenyujin … sccm report hardware inventory

"WebApr 30, 2024 · As expected, purified geosmin synthase yielded the sesquiterpenoids geosmin, germacradienol and germacrene D in the assay mixture upon incubation with FPP (S2 Fig and S3 Fig), whereas the monoterpene limonene was formed when GPP was added to limonene synthase containing crude extracts (S4 Fig and S5 Fig). " - Germacrene synthase

Germacrene synthase

Geosmin Biosynthesis. Streptomyces coelicolor Germacradienol/Germacrene ...

WebJun 26, 2024 · Zager and colleagues reported the identities of CsTPS16, a germacrene B synthase, and CsTPS20, a hedycaryol synthase. We were able to confirm the activities of both of these enzymes as germacrene B and hedycaryol synthases, respectively, using CsTPS16 and CsTPS20 cloned from cv PK [CsTPS16(PK) and CsTPS20(PK), … WebDespite their importance, only four terpene synthases have been identified from the Zingiberaceae family: (+)-germacrene D synthase and (S)-β-bisabolene synthase from ginger rhizome, and α-humulene synthase and β-eudesmol synthase from shampoo ginger (Zingiber zerumbet) rhizome. We report the identification of 25 mono- and 18 …

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WebNeither germacrene A nor any other intermediate is released from sesquiterpene synthase active sites during normal catalysis. Evidence to support the intermediacy of germacrene A has been derived from investigations of aristolochene synthases from Aspergillus terreus and Penicillium roquefortii (Cane, D. E. Chem. Rev. 1990, 90, 1089-1103 and ... WebAug 1, 1998 · The biosynthesis of germacrene C by a homogenate of immature seeds of Kadsura japonica has been reported (Morikawa et al., 1971), as well as the partial …

WebMar 1, 2024 · Isolation and characterization of two germacrene A synthase cDNA clones from chicory. Plant Physiol., 129 (1) (2002), pp. 134-144. CrossRef View in Scopus Google Scholar. Cai et al., 2024. P. Cai, X. Wu, J. Deng, et al. Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia ... WebApr 1, 1999 · Formation of (-)-germacrene D involves a stereospecific 1,3-hydride shift of H-1si of farnesyl diphosphate. Both products are formed from a common intermediate. The enzyme mediates a key step in the biosynthesis of geosmin (see EC 4.1.99.16), a widely occurring metabolite of many streptomycetes, bacteria and fungi.

WebJul 12, 2016 · Analogously, germacrene C synthase from Solanum lycopersicum clone pLE11.3 was also shown to accept more than one substrate, producing germacrene C (64%), germacrene A (18%), germacrene B (11%), and germacrene D (7%) with FDP, and limonene with GDP (Colby et al., 1998). TABLE 1. WebMar 3, 2015 · Germacrene A synthase is a two-domain, α−β-terpenoid synthase with the active center in α-domain (C-terminus, 234–558 AA) exhibiting class I terpene synthase activity (Christianson, 2008; Rajabi et al., 2013). β-domain in N-terminus (32–245 AA) has lost the catalytic activity in mono- and sesquiterpene synthases, and seems to play a ...

WebFeb 3, 2024 · EC 4.2.3.-. Acting on phosphates. Date. Modify. 2024-04-02. While germacrene A is an enzyme-bound intermediate in the biosynthesis of a number of phytoalexins, e.g. EC 4.2.3.9 from some species and EC 4.2.3.21, it is the sole sesquiterpenoid product formed in chicory. ENZYME.

WebAug 11, 2024 · Germacrene A (GA) is a key intermediate for the synthesis of medicinal active compounds, especially for β-elemene, which is a broad-spectrum anticancer drug. … sccm report inactive computersWebDec 16, 2008 · Sesquiterpene synthase that catalyzes the formation of germacrene A. Can use farnesyl diphosphate as substrate, but not geranyl diphosphate or geranylgeranyl diphosphate. Beta-elemene, the initially measured product in the assay, is derived nonenzymatically from germacrene A. 1 publication sccm reporting logsWebSep 20, 2012 · Highlights This manuscript is firstly characterized for germacrene A synthase gene in Citrus species. The gene identification of mono- or sesqui-terpene … sccm report free disk spaceWebJan 7, 2024 · The sesquiterpene germacrene A is a direct precursor of ß-elemene that is a major component of the Chinese medicinal herb Curcuma wenyujin with prominent … sccm reporting configurationWebJul 1, 2006 · The N-part of geosmin synthase catalyzes the cyclization FPP to germacradienol and germacrene D the C-part catalyzes cyclization and fragmentation of germacradienol into geosmin (Giglio et al ... sccmreporting.logWebTerpene synthase genes involved in the biosynthesis of the major monoterpenes and sesquiterpenes routinely assayed by cannabis testing laboratories were identified and functionally evaluated. ... (nerolidol/linalool synthases), CsTPS16CC (germacrene B synthase), and CsTPS20CT (hedycaryol synthase). This study lays the groundwork for … sccm reporting powerbiWebJul 5, 2004 · --germacrene D synthase: BLAST Add. Sequence: Expand table. Expression. Tissue specificity. Expressed in flowers. Detected in stems, young leaves and tendrils. Developmental stage. Expressed in flower buds and detected in open pre-anthesis flowers, flowers after anthesis and in early stages of fruit onset. sccm reporting server requirements