WebResults: In this way Cy5.5 is measurable at 488-nm and 633-nm excitation. Further, with the two different filters it is possible to distinguish Cy5 from Cy5.5 in the same detection … WebCommonly used filter sets for multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed for DAPI / Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640 nm). In our hands the best fluorescent proteins for imaging with this set are ...
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WebNote the prevalent co-localization of GFP and Cy5 in the NESPylRS AF sample, contrary to the appearance of additional nonspecific nucleolar signal in PylRS AF expressing cells (highlighted by arrows). WebNov 5, 2006 · Confocal scanning laser microscopy (CSLM) was used to visualise the spatial location of foulants during the fouling of Q Sepharose FF matrix in finite batch experiments and for examining the subsequent effectiveness of clean-in-place (CIP) treatments in cleaning the heavily fouled beads. ... PicoGreen and Cy5.5, for labelling genomic … brenham high school map
Visualising fouling of a chromatographic matrix using confocal …
WebApr 3, 2024 · We next investigated the uptake of Cy5.5-AuNPs in fixed brains sections, sampling brains from mice sacrificed 30 days after ICM delivery of Cy5.5-AuNPs. Using confocal microscope, we acquired … WebThe most popular lasers for confocal microscopy are air-cooled argon and krypton-argon ion lasers, the new blue diode lasers, and a variety of helium-neon systems. Collectively, these lasers are capable of providing excitation at ten to twelve specific wavelengths between 400 and 650 nanometers. WebCy5 HYQ - The Cy5 HYQ filter combination for red-wavelength excitation is designed to provide high-energy excitation of the fluorophore Cy5, although the wide excitation range (yellow-orange to red) allows the set to be utilized with a variety of fluorochromes. brenham high school transcript